The molecular analysis service provides equipment access and training for biochemical and molecular biology analyses essential to study cancer and ageing at the molecular, cellular or tissular levels. It includes molecular imager for immunoblot, quantitative real-time PCR machines, plate readers for absorbance, fluorescence and luminescence.
Members
Equipment
2 qPCR QS3 (Thermofisher) with 96-wells block
Quantitative real-time PCR
1 Spectrophotometer Nanodrop 2000
UV-Vis Spectrophotometer for microvolumes
1 Qubit 2.0 (Life Technologies)
Fluorometer for DNA, RNA or protein dosage
- – 1 Plate reader Centro-XS (Berthold) (luminescence)
- – 1 Plate reader Tristar (Berthold) (absorbance, fluorescence)
- – 1 Plate reader SpectroStarnano (absorbance)
- – 1 Molecular Imager Fusion Solo (chemiluminescence)
- – 1 Molecular Imager ImageQuant 800 (Cytiva: chemiluminescence, fluorescence, UV and infrared fluorescence)
Getting started/Access
Anyone wishing to use a device must contact Christian Baudoin christian.baudoin@univ-cotedazur.fr. Training on the device will be provided. Use of the devices is subject to training and the signing of the platform’s user charter.
Access & Booking
With the exception of next-generation sequencing, Genomics Core Facility equipment is open-access after signature of the charter and online mandatory reservation. A small fee to cover the operating costs is charged by IRCAN to research teams based on their use.
qPCR cost
Consumables (plastics and kits) are not provided by the Service and need to be directly purchased by users. The fees are related to the use of the qPCR machines (contribution to the maintenance) and are billed to the teams based on their use.