The Pasteur-IRCAN Molecular and Cellular Imaging Core Facility (PICMI) gathers the scientific and technical expertise and state of the art devices required for scientific projects using imaging techniques.
We provide services ranging from simply accessing our facilities to performing your imaging experiments. You can thus choose between :
- Becoming an autonomous user thanks to training sessions adapted to your experimental needs and a follow up on the apparatus used. You can then use it according to your needs and manage your schedule.
- Having our platform operators perform your sample preparation and image acquisitions to gain time and benefit from their expertise.
You can reserve time on this or any other platform at IRCAN through out online booking system
Equipment
« LSM » Upright Confocal Microscope LSM ZEISS
4 solid states lasers (488nm, 633nm, 543nm et 405nm), HAL100 illumination lamp, polarized, analyzers et Wollaston prism for DIC, objectives 10x/0,3/EC Plan-NEOFLUAR, 20x/0,8/Plan-APOCHROMAT, 40x/0,95/PlanAPOCHROMAT: 63x/1,40/oil/DIC/Plan-APOCHROMAT, one PMT detector, 1 PC station for images acquisition and analysis, ZEN software
« LSM880 » inverted Confocal Microscope LSM 880 ZEISS
laser Argon ion (458nm, 488nm, 514nm), laser HeNe (633nm), laser HeNe (543nm), solide state laser 405nm, HAL100 lmap, analyzers et prism Wollaston for DIC, Motorized x, y et z with PIEZZO, objectives 10x/0,3/EC Plan-NEOFLUAR, 20x/0,8/Plan-APOCHROMAT, 40x/0,95/Plan-APOCHROMAT : 63x/1,40/oil/DIC/Plan-APOCHROMAT, two PMT detectors, spectral detector 32 GaAsp, Fast AiryScan, CO2and temperature controller, one PC station for acquisition et one PC station for image analysis, ZEN software
« LSFM » Light Sheet Fluorescent MicroscopyZ.1 Zeiss
4 solid states lasers (405nm, 488nm, 543nm et 633nm), two sCMOS PCO.edge cameras, illumination objectives 5X and 10X; Acquisition objectives 5X, 20X et 20X for transparisation. Incubation chamber 5x,20x n=1.33, 5x n=1.45 and 20x n=1.45 for transparisation. CO2and temperature controller, one PC station for acquisition et one PC station for image analysis, ZEN software
« DV » Time Lapse – DeltaVisionOlympus
4 diodes 405nm, 488nm, 543nm, 632nm, HAL100 lamp, polarized, analyzer and prism Wollaston for DIC, motorized x, y et z, objectives OLYMPUS 20x/0,75, 60x/1,42/oil, 100x/1,40/oil, CCD sCMOS monochromatic camera, 2048×2048 pixels, 6,45μm x 6,45μm, 15 Bit, CO2and temperature controller, one PC station for acquisition with deconvulation
« CytoGen » –Axio Imager Z2 Microscope ZEISS
lampe X-Cite series 120Q, motorized x, y et z, with 8 slides older, objectives 10x/0,45/Plan-APOCHROMAT, 20x/0,5/ EC PlanNEOFLUAR, 40x/0,95/Plan-APOCHROMAT, 63x/1,40/oil/DIC/PlanAPOCHROMAT, 100x/1,40/oil/DIC/Plan-APOCHROMAT, HAD CCD CoolCube monochromatic camera 1360×1024 pixels, 6,45μm x 6,45μm, 12 Bit, 1 PC station for images acquisition and analysis, MetaSystems software
« MetaMorph » Time Lapse – Axiovert 200M Microscope ZEISS
lamp HXP 120C, HAL100 lamp, polarized, analyzers and prism Wollaston for DIC, motorized x, y et z, objectives 5x/0,12/ A Plan, 10x/0,25/A Plan, 20x/0,4/LD Plan NEOFLUAR, 32x/0,4/ LD A Plan, 40x/1,30/oil/FLUAR, 63x/1,40/oil/DIC/Plan APOCHROMAT, EMCCD monochromatic camera Photometrics CASCADE II, 1024×1024 pixels, 13μm x 13μm, 16 Bit, CO2and temperature controller, one PC station for acquisition and for image analysis MetaMorph software
« HD » Time Lapse – Axio Observer Z1 Microscope ZEISS
Lamp HXP 120C, Colibri (leds) illumination (excitation at 365nm, 470nm, 555nm, 625nm), HAL100 lamp, motorized x, y et z, Definite Focus, objectives 2,5x/0,75/PlanNEOFLUAR, 10x/0,3/Plan-NEOFLUAR, 20x/0,4/Plan-NEOFLUAR, 20x/0,8/Plan-APOCHROMAT, 40x/0,6/Plan-NEOFLUAR, 40x/0,75/ EC PlanNEOFLUAR , Optovar (numerical zoom), AxioCam Icc1 ZEISS colored camera, 1388×1038 pixels, 4,65μm x 4,65μm, 8Bit, sCMOS ORCA-Flash 4.0 LT Hamamatsu camerac C11440 2048×2048 pixels, 6,5 μm x 6,5 μm, 16bit, CO2and temperature controller, one PC station for acquisition and for image analysis ZEN2 software.
Five PC controllers for image analysis:
One station for image analysis with MetaMorph
One station for image analysis LSM (ZEN soft)
One station for image analysis LSFM
One station for image analysis LSM 880
One station for image analysis and deconvolution DeltaVision
One station for image analysis with Imaris software
One station for image analysis CytoGen
Rates
Below the Rates ( excl tax) for using the equipment of the PICMI facility (as of 01. 2019)
| Prestations | Unité d’œuvre | Site | Académiques | Privés |
| Métamorph fixe | heure | 3,38 €* | 22,88 € | 28,60 € |
| Métamorph live | heure | 5,08 €* | 24,98 € | 31,22 € |
| HD Fixe | heure | 4,4 €* | 24,14 € | 30,17 € |
| HD live | heure | 6,1 €* | 26,24 € | 32,80 € |
| CYTOGEN | heure | 5,49 €* | 25,48 € | 31,85 € |
| Confocal | heure | 15,83 €* | 54,36 € | 67,95 € |
| LSM 880 fixe | heure | 17,59 €* | 21,72 € | 27,15 € |
| LSM 880 live | heure | 18,59 €* | 22,96 € | 28,70 € |
| feuillet de lumière** | créneau | 80,00 €* | 151,88 € | 159,84 € |
| DM4000 | heure | 2,5 €* | 7,72 € | 9,65 € |
| DV fixe | heure | 14,27 €*** | non contacter | non contacter |
| DV live | heure | 15,97 €*** | non contacter | non contacter |
| Imaris | heure | non contacter | non contacter | non contacter |
| AFM | jour | 103,69 €*** | non contacter | non contacter |
* : applicables aux laboratoires affiliés à l’université de Nice et aux unités Inserm
** : créneau de 4 h incompressible avec l’installation par la plateforme d’un objectif. Si plusieurs objectifs sont nécessaires nous consulter.
*** : applicables aux laboratoires affiliés à l’université de Nice et aux unités CNRS