The Pasteur-IRCAN Cellular and Molecular Imaging core facility (PICMI) expertise covers multi-scale imaging from the molecule to the organism in the fields of cancer and aging.
The PICMI platform, located at the IRCAN institute, PEMED building K, CHU Pasteur, 30 avenue voie Romaine, 06000 Nice, France, is part of MICA multi-site platform (Microscopie Imagerie Cytométrie Azur) gathering imaging facilities of Institut de Biologie de Valrose (iBV), Centre Méditerannéen de Médecine Moléculaire (C3M), IMEV, le Centre Commun de Microscopie Appliquée (CCMA), and Institut Sophia Agrobiotech (ISA INRA).
PICMI gathers equipment in advanced light microscopy, in confocal microscopy and in atomic force microscopy (see specific section).
PICMI provides :
- state of the art technologies and expertise in photonic, AFM and image analysis
- training assistance and advice to users. We offer specific training for users to be autonomous on the platform.
- Collaborate on science and technology projects
The platform is open to all researchers, both academics and non-academics from the scientific community.
Members
- Soline ESTRACH CR Inserm
- Frédéric BRAU, IR CNRS
- Aaron Mendez Bermudes. IR INSERM
- Serge Bauwens, IR CNRS
- Laurent Grosse, IR CNRS
- Sabrina PISANO, IR INSERM
Equipment
Upright Laser-Scanning Confocal Microscope LSM 800 (Carl Zeiss)
- Lasers lines : 405, 488, 561, 633 nm
- Objectives:
- EC Plan-Neofluar 10x/0,3
- Plan-Apochromat 20x/0.8
- Plan-Apochromat 40x/0,95
- Plan-Apochromat 63x/1.4 DIC
- 3 PMT detectors including 1 GAsP
- XY motorized stage
- ZEN Blue software
Inverted Laser-Scanning Confocal Microscope LSM 880 Airy scan (Carl Zeiss)
- Lasers lines : Diode 405nm, Argon : 458nm, 488nm, 514nm, DPSS 561nm, HeNe 633nm
- Objectives:
- EC Plan-Neofluar 10x/0,3
- Plan-Apochromat 20x/0.8
- Plan-Apochromat 40x/1.4
- 2 PMT detectors, 1 spectral detector 32 channels GaAsp, 1 Fast AiryScan
- XY motorized stage with Z piezo stage
- CO2 and temperature controler
- ZEN Black software
Light Sheet Fluorescent Microscope Light Sheet Z.1 (Carl Zeiss)
- Lasers lines : 405nm, 488nm, 561nm, 633nm
- 2 sCMOS PCO.edge cameras
- Objectives : Illumination : 5X and 10X
- Acquisition : 5X, 20X and 20X for transparisation
- Incubation chamber 5x,20x n=1.33, 5x n=1.45 and 20x n=1.45 for transparisation.
- CO2 and temperature controller,
- ZEN Blue software
Video-microscope and TIRFM DeltaVision Elite (GE- Leica Microsystems)
- 4 diodes 405nm, 488nm, 543nm, 632nm,
- HAL100 lamp,
- Polarized, analyzer and prism Wollaston for DIC, motorized x, y et z,
- objectives OLYMPUS 20x/0,75, 60x/1,42/oil, 100x/1,40/oil,
- CCD sCMOS monochromatic camera, 2048×2048 pixels,
- 6,45μm x 6,45μm, 15 Bit,
- CO2 and temperature controller,
- one PC station for acquisition with deconvolution
Axio Imager Z2 Microscope -8 slide holder (Carl Zeiss)
lamp HXP 120C, motorized x, y et z, with 8 slides older, objectives 10x/0,45/Plan-APOCHROMAT, 20x/0,5/ EC PlanNEOFLUAR, 40x/0,95/Plan-APOCHROMAT, 63x/1,40/oil/DIC/PlanAPOCHROMAT, 100x/1,40/oil/DIC/Plan-APOCHROMAT, Camera Axiocam 705 mono, Zen blue software.
HAD CCD CoolCube monochromatic camera 1360×1024 pixels, 6,45μm x 6,45μm, 12 Bit, 1 PC station for images acquisition and analysis, MetaSystems software
xVideo-microscope Axiovert 200M (Carl Zeiss)
lamp HXP 120C, HAL100 lamp, polarized, analyzers and prism Wollaston for DIC, motorized x, y et z, objectives 5x/0,12/ A Plan, 10x/0,25/A Plan, 20x/0,4/LD Plan NEOFLUAR, 32x/0,4/ LD A Plan, 40x/1,30/oil/FLUAR, 63x/1,40/oil/DIC/Plan APOCHROMAT, EMCCD monochromatic camera Photometrics CASCADE II, 1024×1024 pixels, 13μm x 13μm, 16 Bit, CO2 and temperature controller, one PC station for acquisition and for image analysis MetaMorph software
Video-microscope Axio Observer Z1 (Carl Zeiss)
Lamp HXP 120C, Colibri (leds) illumination (excitation at 365nm, 470nm, 555nm, 625nm), HAL100 lamp, motorized x, y et z, Definite Focus, objectives 2,5x/0,75/PlanNEOFLUAR, 10x/0,3/Plan-NEOFLUAR, 20x/0,4/Plan-NEOFLUAR, 20x/0,8/Plan-APOCHROMAT, 40x/0,6/Plan-NEOFLUAR, 40x/0,75/ EC PlanNEOFLUAR , Optovar (numerical zoom), AxioCam Icc1 ZEISS colored camera, 1388×1038 pixels, 4,65μm x 4,65μm, 8Bit, sCMOS ORCA-Flash 4.0 LT Hamamatsu camerac C11440 2048×2048 pixels, 6,5 μm x 6,5 μm, 16bit, CO2 and temperature controller, one PC station for acquisition and for image analysis ZEN2 software.
Getting Access
The access to PICMI facility depends first on access to the PEMED building. First step get the clearance and the safety formation for this building, thus getting the badge activated. Then follow theoretical formation on light microscopy then choose the material you will need to use for your experiment and follow specific technical training by one of the PICMI member. All those steps will be validated, and the platform will keep the log of the users properly trained.