Expertise and Service offer/ Applications
The platform provides comprehensive histology services, covering all steps from sample embedding to staining (including immuno-labeling):
- Processing of tissues from a wide variety of species and types
- Paraffin embedding and microtome sectioning (standard or serial)
- Frozen tissues embedding (OCT) and cryostat sectioning
- Routine histological stains (Hematoxylin & Eosin, Picro Sirius Red)
- Immunohistochemical (IHC) labeling
Members
Laetitia Seguin, CRCN Inserm, scientific head
Clément Molina, TCS Inserm, technical head
Equipment & reagents
Sample Preparation
- Vacuum tissue dehydration carousel (TP 1020, Leica)
- Paraffin embedding station (Histocore Arcadia, Leica)
Sectioning
- Two microtomes with a water bath, for thin sectioning of fixed, paraffin-embedded tissues (Rm2255, Leica Biosystems).
- One microtome HM 355S + STS (Niagara system, Thermo) optimized for fine sections of small, precious samples while preserving biological integrity.
- Cryostat (NX50, Thermo) for frozen sections.
- Vibratome (HM650V, CU65, Thermo) for precise sectioning of fresh tissues without freezing or embedding.
- Binocular stereomicroscope.
Additional Resources
- -80 °C freezer (HDE30086FV)
- Tissue cassettes
- OCT
Stains & Reagents
- Hematoxylin & Eosin (H&E)
- Picro Sirius Red
- Prolong Gold antifade (invitrogen)
- Elite ABC kit, Peroxidase (PK-6100, vector lab)
- MOM kit (BMK-2202, Vector lab)
- DAB
- Avidin-Biotin kit
Getting started/ Access
- Mandatory training is required before using any equipment.
- For assistance or training requests, please contact:
- Laetitia Seguin, laetitia.seguin@univ-cotedazur.fr
- Clément Molina, clement.molina@univ-cotedazur.fr
- Then, reservation is required for equipment usage via the booking website.
Publications
- Tissot FS, Estrach S, Seguin L, Cailleteau L, Levy A, Aberdam D, Féral CC. Functional transfer of integrin co-receptor CD98hc by small extracellular vesicles improves wound healing in vivo. Matrix Biol. 2025 Feb;135:99-105. doi: 10.1016/j.matbio.2024.12.006. Epub 2024 Dec 12. PMID: 39674554. Iltis C,
- Moskalevska I, Debiesse A, Seguin L, Fissoun C, Cervera L, Moudombi L, Ardin M, Ferrari A, Eliott C, Pisani D, Ottaviani A, Bourinet M, Luci C, Gual P, Makulyte G, Bernard D, Durandy M, Duret LC, Hamidouche T, Kunz S, Croce O, Delannoy C, Guérardel Y, Allain F, Hofman P, Benarroch-Popivker D, Bianchini L, Dadone-Montaudie B, Cosson E, Guglielmi J, Pourcher T, Braud VM, Shkreli M, Pers YM, Jorgensen C, Brondello JM, Féral CC, Michallet MC, Gilson E, Cherfils-Vicini J. A ganglioside-based immune checkpoint enables senescent cells to evade immunosurveillance during aging. Nat Aging. 2025 Feb;5(2):219-236. doi: 10.1038/s43587-024-00776-z. Epub 2024 Dec 27. PMID: 39730825; PMCID: PMC11839482.
- Ropert B, Bannwarth S, Genin EC, Vaillant-Beuchot L, Lacas-Gervais S, Hounoum BM, Bernardin A, Dinh N, Mauri-Crouzet A, D’Elia MA, Augé G, Lespinasse F, Di Giorgio A, Meira W, Bonnefoy N, Monassier L, Schiff M, Sago L, Kilinc D, Brau F, Redeker V, Bohl D, Tribouillard-Tanvier D, Procaccio V, Azoulay S, Ricci JE, Delahodde A, Paquis-Flucklinger V. Nifuroxazide rescues the deleterious effects due to CHCHD10-associated MICOS defects in disease models. Brain. 2024 Oct 30:awae348. doi: 10.1093/brain/awae348. Epub ahead of print. PMID: 39478664.
- Janho Dit Hreich S, Juhel T, Leroy S, Ghinet A, Brau F, Hofman V, Hofman P, Vouret-Craviari V. Activation of the P2RX7/IL-18 pathway in immune cells attenuates lung fibrosis. Elife. 2024 Feb 1;12:RP88138. doi: 10.7554/eLife.88138. PMID: 38300690; PMCID: PMC10945561.
- Mousset A, Lecorgne E, Bourget I, Lopez P, Jenovai K, Cherfils-Vicini J, Dominici C, Rios G, Girard-Riboulleau C, Liu B, Spector DL, Ehmsen S, Renault S, Hego C, Mechta-Grigoriou F, Bidard FC, Terp MG, Egeblad M, Gaggioli C, Albrengues J. Neutrophil extracellular traps formed during chemotherapy confer treatment resistance via TGF-β activation. Cancer Cell. 2023 Apr 10;41(4):757-775.e10. doi: 10.1016/j.ccell.2023.03.008. PMID: 37037615; PMCID: PMC10228050.
- Pouchin P, Zoghlami R, Valarcher R, Delannoy M, Carvalho M, Belle C, Mongy M, Desset S, Brau F. Easing batch image processing from OMERO: a new toolbox for ImageJ. F1000Res. 2022 Apr 5;11:392. doi: 10.12688/f1000research.110385.2. PMID: 35685190; PMCID: PMC9171289.