The Cytometry Core Facility, Cytomed, is located in the PEMED PCV building (K building CHU Pasteur, Nice). This facility is a member of MICA multi-site platform (Microscopie Imagerie Cytométrie Azur). Cytomed allows functionnal and statistical analysis of cellular populations from tissues or cell lines, through individual cellular caracterizations : membrane, intracellular markers, DNA content… Cytomed provides know-how, knowledge and access to equipments for sample preparation, flow cytometry acquisition and analysis and cell sorting.
Expertise and Service offer/ Applications
- State-of-the-art up to 18 color flow cytometry with high througput automation, cell sorting technologies
- Expertise in cell sorting from cells to small organisms (sorting of large particles from 10µM to 2,000µM including large cell mass (kidney glomerules, pancreatic islet, neurosphere…), fragile cells (adipocytes, neurons…) or small model organisms (Nematostella, zebrafish larvae, nematodes…).
- Assistance, advice, and training to the users to design their multi-color panels, sorting strategies, sample preparation from tissue dissociation to magnetic beads pre-sorting…
- In-flow imaging for cell caracterisation and functional analysis : cell cycle, apoptosis, ploïdy, nuclear translocation…
- Data analysis with dedicated softwares : FACS Diva 6, FlowJo, CytExpert, IDEAS
- Dedicated spaces for cell culture, preparation, staining, flow cytometry acquisition and analysis in L1 and cell sorting in L2
Cytomed is involved in regional and national diffusion of knowledge and know-how through MICA trainings and scientific animation.
Cytomed collaborates with teams from MICA partners and Centre Scientific de Monaco for cell sorting as well as academic teams in Lyon and Bordeaux.
Members
Julien Cherfils-Vicini (PhD) as Scientific head of CytoMed
Ludovic Cervera, Inserm IE, at 50%
Emanuela Repetto, UCA AI, at 20%
Equipment
Flow cytometers analyzers
Flow cytometer imager ImageStreamX (Merck Millipore / Cytek, 2018)
4 lasers: 405, 488, 561, 633nm
2 CCD TDI cameras
FSC/SSC – 10 fluorescent channels
3 objectives (20X, 40X, 60X) – with extension field module
Flow cytometer CytoFlex (Beckman Coulter, 2019)
5 lasers (375, 405, 488, 561, 633nm)
FSC/SSC – 20 fluorescent channels
Cell Sorters
Cell sorter FACS Aria III (BD Biosciences, 2012)
5 lasers : 375, 405, 488, 561, 633nm
FSC/SSC – 18 fluorescent channels
Multi-cellular/small organisms Cell sorter BioSorter (UnionBIometrica, 2013)
2 lasers: 488,561nm
5 fluorescent channels
Culture Room and Sample preparation
Incubators
Multi-Macs separator plus
Gentle Macs tissue dissociator
PSM
Fluorescent microscope
Centrifuges
Getting started/ Access
Tell us about your project!
Teams wishing to use the equipment or perform a service must first contact the Cytomed staff by sending a message to cytomed@univ-cotedazur.fr, contact a member of staff directly, or come and talk to us in PEMED. Users should specify their request by explaining their biological question to help us decide together on the most suitable approach.
For CytoFlex, ImagestreamX, Seahorse, GentleMACS, MultiMACS: each user is trained to use these apparatus. After training, validation by CytoMed staff and agreement by the user of CytoMed rules, users will have access to online booking of the systems. The reservation procedures will be specified during the initial training.
For cell sorting (BioSorter ou Aria III) that required CytoMed staff: after a request by email, a first appointment is fixed to discuss about sample preparation, staining and panel design. When the user is ready, a schedule is fixed with the staff to perform the sort. Sorters are not accessible autonomously.
Publications
A ganglioside-based immune checkpoint enables senescent cells to evade immunosurveillance during aging.
Iltis C, Moskalevska I, Debiesse A, Seguin L, Fissoun C, Cervera L, Moudombi L, Ardin M, Ferrari A, Eliott C, Pisani D, Ottaviani A, Bourinet M, Luci C, Gual P, Makulyte G, Bernard D, Durandy M, Duret LC, Hamidouche T, Kunz S, Croce O, Delannoy C, Guérardel Y, Allain F, Hofman P, Benarroch-Popivker D, Bianchini L, Dadone-Montaudie B, Cosson E, Guglielmi J, Pourcher T, Braud VM, Shkreli M, Pers YM, Jorgensen C, Brondello JM, Féral CC, Michallet MC, Gilson E, Cherfils-Vicini J.
Nat Aging. 2025 Feb;5(2):219-236. doi: 10.1038/s43587-024-00776-z. Epub 2024 Dec 27. PMID: 39730825; PMCID: PMC11839482.